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用途
sufficient for 500 fluorometric tests (microplate readers)
檢測方法
fluorometric
相關疾病
cancer
儲存溫度
−20°C
一般說明
由于电子传递链上酶的活动，线粒体在其膜上产生电位。在细胞凋亡过程中，线粒体膜电位（MMP）的崩溃与线粒体通透性转换孔的开放一致，导致细胞色素c 释放到细胞质中，进而引发细胞凋亡级联反应的其他下游事件。
應用
线粒体膜电位试剂盒已用于测量线粒体膜电位。
適合性
本试剂盒适于检测哺乳动物细胞中的线粒体跨膜电势差(MMP)，并可用于在酶标仪上筛选凋亡抑制剂及激活剂等。
原則
该试剂盒利用 JC-10，一种优于JC-1的替代品，用于测定细胞中MMP的损失。尽管 JC-1在许多实验室中被广泛使用，但是当其在较高浓度下使用时，其水溶性差通常会导致水性缓冲液沉淀。在较高浓度下，JC-10 表现出比 JC-1 更高的水溶性。与JC-1 类似，JC-10是一种阳离子亲脂性染料，其浓缩并在具有极化线粒体膜的细胞线粒体中形成可逆的红色荧光 JC-10 聚集体（λex = 540/λem = 590 nm）。在凋亡细胞中，MMP 崩溃导致 JC-10 无法保留在线粒体中，并使染料返回到单体绿色荧光形式（λex = 490/λem = 525 nm）。该试剂盒可用于监测细胞凋亡和筛选凋亡抑制剂和激活剂。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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