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一般說明
内质网(ER)是由互连的小管、小泡和扁囊组成的网络。其在各种细胞特殊功能中发挥着重要作用，包括蛋白质合成、钙隔离、类固醇生产、糖原储备和生产、膜蛋白嵌入等。
應用
内质网分离试剂盒含有制备各种纯度的ER所需的所有试剂：粗面ER（微粒体）、Ca2+ 沉淀粗面ER (RER) 富集微粒体，以及密度梯度纯化所得的粗面ER (RER)和滑面ER (SER)。ER分离过程可采用细胞色素c还原酶检测试剂盒测定NADPH细胞色素c还原酶的活性进行监测（货号：CY0100）。这种酶是一种ER膜蛋白，常被用作ER标志物。分离所得的ER可用于研究P450系统和异源物代谢、脂质代谢，也可用于回收ER膜和腔蛋白。该试剂盒以采用大鼠肝脏、肾脏和脑，小鼠肝脏，兔肝脏、肾脏、脾、心脏和脑，以及Jurkat和HeLa细胞系进行过测试。
内质网分离试剂盒用于从哺乳动物组织或细胞培养物中有效富集功能性内质网（ER）。 分离出的ER可用于蛋白质合成、降解和代谢途径分析。 ER代谢异常包括发生于儿童的肉碱乙酰转移酶缺乏症、囊性纤维化和许多保留更多ER蛋白的病症。 适用于最多50 g的组织或约20 mL的包装组织。
特點和優勢
- 专为研究规模应用配制的提取试剂 - 节省时间并减少浪费
- 包含氯化钙溶液 - 无需超速离心即可快速简单地沉淀粗糙ER
- 产生功能完整的细胞器 - 生成的 ER 适用于功能研究、脂质代谢和蛋白质分析
- 与用于结构确认的产品相兼容 - 与细胞色素 C 还原酶 (CY0100) 配套试剂盒测试一起轻松确认完整性
- 可用于从动物软组织和培养细胞中分离ER
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Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Xin Li et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 8265-8282 (2020-04-16)
Dynamin-Related-Protein 1 (DRP1) critically regulates mitochondrial and peroxisomal fission in multicellular organisms. However, the impact of DRP1 on other organelles, especially its direct influence on ER functions remains largely unclear. Here, we report that DRP1 translocates to endoplasmic reticulum (ER)
Jiang-An Yin et al.
Nature, 551(7679), 198-203 (2017-11-10)
The rate of behavioural decline in the ageing population is remarkably variable among individuals. Despite the considerable interest in studying natural variation in ageing rate to identify factors that control healthy ageing, no such factor has yet been found. Here
Rani Ojha et al.
Cancer discovery, 9(3), 396-415 (2018-12-20)
Resistance to BRAF and MEK inhibitors (BRAFi + MEKi) in BRAF-mutant tumors occurs through heterogeneous mechanisms, including ERK reactivation and autophagy. Little is known about the mechanisms by which ERK reactivation or autophagy is induced by BRAFi + MEKi. Here
Jason A Sprowl et al.
Nature communications, 7, 10880-10880 (2016-03-17)
Membrane transporters are key determinants of therapeutic outcomes. They regulate systemic and cellular drug levels influencing efficacy as well as toxicities. Here we report a unique phosphorylation-dependent interaction between drug transporters and tyrosine kinase inhibitors (TKIs), which has uncovered widespread
Yuan Tian et al.
Nature communications, 10(1), 3391-3391 (2019-07-31)
Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome that elevates the risk of hepatocellular carcinoma (HCC). Although alteration of lipid metabolism has been increasingly recognized as a hallmark of cancer cells, the deregulated metabolic modulation
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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