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Merck

M7653

Sigma-Aldrich

培养基 199

With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

培养基 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

原始培养基199的修改。Earles′盐被Hanks′盐代替。Hanks′盐使用较低浓度的碳酸氢盐和不同浓度和盐形式的必需无机离子。
培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

重構

补充 0.1 g/L L-谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Etienne de Coulon et al.
iScience, 24(2), 102041-102041 (2021-02-04)
A comprehensive understanding of mechano-electrical coupling requires continuous intracellular electrical recordings being performed on cells undergoing simultaneously in vivo like strain events. Here, we introduce a linear strain single-cell electrophysiology (LSSE) system that meets these requirements by delivering highly reproducible unidirectional
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Emilie Brûlé et al.
Journal of the Endocrine Society, 5(4), bvab023-bvab023 (2021-04-03)
Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene result in central hypothyroidism, often associated with macroorchidism. Testicular enlargement in these patients might be caused by increases in follicle-stimulating hormone (FSH) levels, as IGSF1 has been proposed to
Yining Li et al.
The Journal of biological chemistry, 292(6), 2301-2314 (2016-12-21)
Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.
Anna Drożdż et al.
Pharmaceutics, 12(9) (2020-09-17)
Recent years have brought great focus on the development of drug delivery systems based on extracellular vesicles (EVs). Considering the possible applications of EVs as drug carriers, the isolation process is a crucial step. To solve the problems involved in

商品

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

相关内容

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

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