When reconstituted and filter sterilized, this medium is expected to have a similar shelf-life as prepared 1X Medium 199 with Hanks salts and L-glutamine. See product 51322C for the prepared liquid medium. This product has an expiration date of 1 year from the date of quality release. Upon the addition of serum or other heat-sensitive components, the shelf life would be limited to 1 - 2 weeks at 2 - 8°C. Freeze/thaw cycles may result in precipitate formation.
价格与库存信息目前不能提供
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产品名称
培养基 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
表单
powder
技术
cell culture | mammalian: suitable
组分
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
运输
ambient
储存温度
2-8°C
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一般描述
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
应用
培养基199已用于：
- 测试和确定传感器在复杂环境中的pH灵敏度
- 作为卵母细胞采集的培养基
- 作为杜氏利什曼虫（LdBOB）原体培养基的组分，用于培养杜氏利什曼虫
- 内脏脂肪组织外植体
数量
按照每升培养基10.6克粉末配制。
重悬
补充0.35 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
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Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success
Mohammadhossein Dabaghi et al.
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In many biological systems, pH can be used as a parameter to understand and study cell dynamics. However, measuring pH in live cell culture is limited by the sensor ion specificity, proximity to the cell surface, and scalability. Commercially available
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
-
Once prepared, how long can the media stay refrigerated? Can it be frozen?
1 answer-
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