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M2154

Sigma-Aldrich

培养基 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

M199 Medium, TCM 199

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

培养基 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

400

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

pH值

7.0-7.6 (25 °C, 209 g/L)

成分

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

應用

培养基199已用于：
  • 培养一岁Speckle Park母牛来源的瘤胃上皮细胞
  • 作为成熟培养基组分，培养牛卵巢来源的卵丘卵母细胞复合体(COC)
  • 作为杜氏改良Eagle培养基(DMEM)组分，维持新生大鼠心肌细胞(NCM)生长

重構

补充 0.1 g/L L-谷氨酰胺。

也與該產品經常一起購買

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補充物

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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E Held-Hoelker et al.
Theriogenology, 96, 145-152 (2017-05-24)
Lipid accumulation is associated with reduced embryonic quality, causing limited survival after cryopreservation. Therefore, in the present study we aimed to reveal the effects of supplementation of a lipid reducing agent, l-carnitine and the removal of fatty acids during in vitro
C Kent-Dennis et al.
Journal of dairy science, 104(1), 1002-1017 (2020-11-03)
Inflammation of ruminal epithelium may occur during ruminal acidosis as a result of translocation and interaction of ruminal epithelial cells (REC) with molecules such as lipopolysaccharide (LPS). Such inflammation has been reported to alter cellular processes such as nutrient absorption
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Nigel J Brand et al.
Methods in molecular biology (Clifton, N.J.), 633, 113-124 (2010-03-06)
We describe a method of isolating and maintaining primary cultures of mouse neonatal cardiac myocytes (NCM). This is derived from the well-established procedure for making NCM cultures from rat neonates by sequential digestion of rat ventricular myocardial pieces using a
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Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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