H9661

肝细胞生长因子 人

HGF, recombinant, expressed in NSO cells, suitable for cell culture

• 别名: HGF, 散射因子, 肝素A
• MDL號碼: MFCD00217422
• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in NSO cells
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: 20-40 ng/mL
• 品質: endotoxin tested
• 分子量: 70-80 kDa by SDS-PAGE (non-reducing)
• 包裝: pkg of 5 and 25 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: <1.0 EU/μg
• UniProt登錄號: P14210
• 儲存溫度: −20°C
• 基因資訊: human ... HGF(3082)

说明

一般說明

Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1. The encoded protein is a mesenchymal-fibroblast derived cytokine.

應用

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

生化／生理作用

HGF′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor). HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy. Upregulation of the HGF gene is observed in advanced stages of cancer, such as pancreatic adenocarcinoma.

肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。

其他說明

NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。

外觀

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

分析報告

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)