The stability of stock solutions of this enzyme has not been evaluated. However, it is cited in Methods in Enzymology, 165, 3-7 (1988) that at concentrations greater than 2 mg of protein per ml it can be stored at -20 °C without loss of hemolytic titer.
推荐产品
生物来源
Staphylococcus aureus
质量水平
表单
lyophilized powder
比活
≥10,000 units/mg protein
包含
sodium citrate buffer as balance
组成
Protein, ~60% Lowry
溶解性
H2O: soluble 0.49-0.51 mg/mL
UniProt登记号
储存温度
2-8°C
基因信息
Staphylococcus aureus ... SAOUHSC_01121(3920722)
应用
α-来自金黄色葡萄球菌的溶血素已被用于:
- 作为电解质溶液的一种成分，用于通过电生理测量测试脂双层中的孔形成[2]
- 测试其在骨髓基质细胞（BMSCs）中的成骨抑制作用[3]
- 制备用于 Biacore 和表面等离子体共振的α溶血素分子印迹聚合物 (MIP)[4]
α-溶血素被用于一项研究，用于测试乳制品大肠杆菌的外排泵和溶血素活性。它还用于测试其对苯扎氯铵的适应性以及环丙沙星对生物膜形成的影响。[5]
生化/生理作用
包装
包装尺寸基于蛋白质含量
单位定义
一个溶血单元是在 37°C 下 30 分钟，然后在 4°C 下冷藏 30 分钟后，悬浮在 pH 7.0 磷酸盐缓冲盐水中含1% 牛血清白蛋白的 1% 兔红细胞有50%溶解。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2
靶器官
Lungs,Blood
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Ankita Pagedar et al.
The Journal of dairy research, 79(4), 383-389 (2012-08-10)
The present study investigates the effect of adaptive resistance to ciprofloxacin (Cip) and benzalkonium chloride (BC) on biofilm formation potential (BFP), efflux pump activity (EPA) and haemolysin activity of Escherichia coli isolates of dairy origin. All the isolates, irrespective of
Gregory J Digby et al.
The Journal of physiology, 586(14), 3325-3335 (2008-05-24)
Signalling by heterotrimeric G proteins is often isoform-specific, meaning certain effectors are regulated exclusively by one family of heterotrimers. For example, in excitable cells inwardly rectifying potassium (GIRK) channels are activated by G betagamma dimers derived specifically from G(i/o) heterotrimers.
Bryan J Berube et al.
Toxins, 5(6), 1140-1166 (2013-07-31)
Staphylococcus aureus secretes a number of host-injurious toxins, among the most prominent of which is the small β-barrel pore-forming toxin α-hemolysin. Initially named based on its properties as a red blood cell lytic toxin, early studies suggested a far greater
Rakez Kayed et al.
The Journal of biological chemistry, 284(7), 4230-4237 (2008-12-23)
Amyloid oligomers are believed to play causal roles in several types of amyloid-related neurodegenerative diseases. Several different types of amyloid oligomers have been reported that differ in morphology, size, or toxicity, raising the question of the pathological significance and structural
Muhmmad Omar-Hmeadi et al.
Traffic (Copenhagen, Denmark), 19(6), 436-445 (2018-03-16)
Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution total internal
相关内容
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
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We suspended in the product H9395 in 12.5 ml of PBS (our usual water-based infection solvent), flash-froze the aliquots in liquid nitrogen, and stored the aliquots at -80 C. There was no apparent issue with resuspension in PBS vs water. However, we observed no hemolytic activity in our in vivo mouse study or an in vitro quantitative hemolysis assay performed with rabbit blood.
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