The solubility of H375, Hyaluronidase from bovine testes, is 100 mg/mL in water. To achieve a higher solubility, it is advised to warm the tube at 37°C and shake it in an ultrasonic bath for a period. The stock solution can be stored at temperatures below -20°C for several months.
价格与库存信息目前不能提供
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生物来源
bovine testis
质量水平
偶联物
conjugate (Glucosaminoglycan)
类型
Type VIII
表单
lyophilized powder
比活
300-1,000 U/mg
分子量
~55 kDa (four subunits of 14 kDa each)
相关疾病
cancer
储存温度
−20°C
基因信息
cow ... HYAL1(515397) , HYAL2(281838)
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生化/生理作用
这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。
单位定义
在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
制备说明
由无菌灌装的I-S型溶液制备。
其他说明
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
实验方案
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the recommended method for reconstituting item H375, Hyaluronidase from bovine testes?
1 answer-
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Good Morning, I our lab we got this product (H3757) and we would like to know how to obtain the package insert since we are not sure how to store it or after open expiration date. Not lot number on the bottle so not sure how to look for it. Thank you
1 answer-
Hyaluronidase from bovine testes may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin. For more information please view the assay below:
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How is product H3757, Hyaluronidase from bovine testes, solubilized?
1 answer-
Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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