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H1136

Sigma-Aldrich

透明质酸酶 来源于 Streptomyces hyalurolyticus

别名:

透明质酸裂解酶 来源于 Streptomyces hyalurolyticus

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About This Item

CAS号:
37259-53-3
EC 号:
4.2.2.1 (BRENDA, IUBMB)
EC 号:
253-430-8
MDL编号:
MFCD00131347
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Streptomyces sp. (S. hyalurolyticus)

质量水平

200

偶联物

conjugate (Glucosaminoglycan)

表单

film (Clear)

包装

ampule of 300 ×  units

储存温度

−20°C

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相关类别

一般描述

透明质酸酶广泛存在于自然界。在人体中，它存在于不少器官和体液中。透明质酸酶见于病毒、噬菌体、真菌和细菌的分泌物。它还存在于线虫和蚂蟥的分泌物中。蛇和蝎子的毒液中会产生透明质酸酶。蜜蜂、蜘蛛、黄蜂、大黄蜂、毛虫、鱼类和蜥蜴也会分泌透明质酸酶。

应用

透明质酸酶（来自Streptomyces hyalurolyticus）已用于：
  • 制备DMEM/F-12（杜氏改良Eagle培养基/营养混合物F-12）培养基，用于从分离肿瘤组织中分离和纯化单细胞
  • 作为消化液组分，用于从睾丸组织获得生殖干细胞

生化/生理作用

透明质酸裂解酶在β-D-GlcNAc-(1→4)-β-D-GlcA键上切割透明质酸，得到3-(4-脱氧β-D-葡萄糖-4-烯基糖基)-N-乙酰基-D-葡糖胺四糖和六糖。与其他透明质酸酶不同，该酶对透明质酸具有特异性，并且对软骨素和硫酸软骨素无活性。
透明质酸酶属于内切糖苷酶，通过配合β-葡糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶等外切糖苷酶完成其内切功能。透明质酸酶调节透明质酸的周转代谢。

单位定义

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

其他说明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000436738
0000436738
0000425385
0000425385
0000419723

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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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