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D7058

Sigma-Aldrich

DirectLoad™ 宽分子量 DNA 标准

ready-to-use marker for DNA electrophoresis

别名:

DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis

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About This Item

分類程式碼代碼:
41105335
NACRES:
NA.25

品質等級

100

形狀

liquid

用途

100 uses

適合性

suitable for electrophoresis (DNA)

儲存溫度

−20°C

相关类别

一般說明

Sigma的DirectLoad宽分子量 DNA 分子量标准包含16个从50 - 10,000 bp并带有规则间隔的片段。10 ul分子量标准可直接上样至琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。

應用

适于通过琼脂糖或聚丙烯酰胺凝胶测定PCR产生的DNA片段的大小。

特點和優勢

  • 可直接上样
  • 易于使用
  • 常见的条带大小

成分

Sigma的DirectLoad™宽分子量 DNA 分子量标准以含有2.5% Ficoll（400型）、0.0125%溴酚蓝以及0.00625%二甲苯蓝的凝胶上样溶液形式提供。

其他說明

含有16个50 - 10,000 bp片段，在琼脂糖凝胶上呈现合理间隔的条带。
为实现最佳分辨率，建议的琼脂糖凝胶浓度为0.75 - 2.0%。

法律資訊

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Melanie P Dautle et al.
Journal of clinical microbiology, 40(2), 414-421 (2002-02-05)
In this study, 83 clinical isolates purified from biofilms colonizing 18 silicone gastrostomy devices (12 "buttons" and six tubes converted to skin level devices) were selected for subtype characterization utilizing genetic analysis. The tubes, previously used for feeding, remained in

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实验方案

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Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

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