D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

• 分類程式碼代碼: 12352200
• NACRES: NA.25

属性

• 形狀: buffered aqueous solution
• 用途: 100 uses (0.1 mg size); 20 uses (20 μg size)
• 適合性: suitable for electrophoresis (DNA)
• 儲存溫度: −20°C

说明

一般說明

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

應用

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

成分

10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA

準備報告

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

其他說明

For optimal resolution, the recommended agarose gel concentration is 0.4%.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable