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一般描述
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
组分
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
法律信息
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
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- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture化学品安全说明书
- W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)化学品安全说明书
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)化学品安全说明书
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
靶器官
Central nervous system
储存分类代码
5.1A - Strongly oxidizing hazardous materials
闪点(°F)
100.4 °F
闪点(°C)
38 °C
Henriette O'Geen et al.
BioTechniques, 41(5), 577-580 (2006-12-05)
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
实验方案
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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