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I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

别名:

辛基苯氧基聚（乙烯氧基）乙醇，支链, 聚氧乙烯辛烷基苯酚醚，分支, 辛基苯基-聚乙烯二醇

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About This Item

线性分子式:
(C2H4O)nC14H22O
CAS号:
9002-93-1
UNSPSC代码:
12352104
NACRES:
NA.31
价格与库存信息目前不能提供

等级

Molecular Biology
for molecular biology

描述

non-ionic

表单

liquid

技术

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

异质活性

DNase and RNase, none detected

SMILES字符串

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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相关类别

一般描述

IGEPAL® CA-630是一种非离子型、非变性去污剂，适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。[1]

应用

IGEPAL® CA-630已用于：
  • 在小鼠肌肉匀浆液的蛋白质印迹分析(WB)中，作为匀浆缓冲液的中性去垢剂[2]
  • 在E8.5-E10.5胚胎的lacZ表达中，用于X-gal染色[3]
  • 加入细胞裂解(CL)缓冲液中，制备RT-qPCR即用型细胞裂解液[4]

其他说明

非离子型、非变性洗涤剂

质量

在化学性质方面，不能与已经退市的 Nonidet P-40 区分开。

注意

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。

法律信息

IGEPAL is a registered trademark of Solvay

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

483.8 °F - closed cup

闪点(°C)

251 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCX2388
MKCW7585
MKCV4219
MKCR5566
MKCR3234

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Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
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实验方案

RIP试剂盒的哺乳动物细胞Ago抗体-RNA免疫沉淀实验方案

使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

相关内容

蛋白质Pull-Down技术

利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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Questions

1–10 of 17 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. In which solvent(s) is Atazanavir Sulfate soluble?

    1 answer

    1. Atazanavir Sulfate is soluble in DMSO: 104mg/mL and EtOH: 20mg/mL.

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  4. What is the storage temperature for this item?

    1 answer

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

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  5. Hi, I am enquiring about the storage temperature of product IGEPAL(R) CA-630 (I8896-100ml). The storage class is listed in the SDS but no temperature upper and lower limits. Would I be able to request this information. Thank you

    1 answer

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, and the Safety Data Sheet (SDS).

      Helpful?

  6. What is the molecular weight of Product No. I8896, IGEPAL® CA-630?

    1 answer

    1. The approximate molecular weight of this product is 603 Daltons. The average EO (ethylene oxide) units per molecule are 9.

      Helpful?

  7. What is the wt/vol % of this IGEPAL reagent? I want to use it for ATAC-seq and in the protocols people always mention the concentration of the stock is 10% wt/vol but I can't find this information on the specifications of this product. Thanks

    1 answer

    1. This product is provided as a neat liquid with a concentration of 100% which can be further diluted. The density of IGEPAL CA-630 is 1.06 g/cm3 at 25 °C. For a 10% wt./vol. solution 9.43 mL IGEPAL is needed per 100 mL of solution.

      The product is generally stable based on the chemical composition and a 10% (w/v) aqueous solution may be stable refrigerated for at least one month. However, it is important to minimize the exposure of the solution to oxygen (keep closed soon after use). The product does have a tendency to oxidize in air forming peroxides through a free radical process. In addition, some bacterial growth may occur which is evidenced by an odor or formation of a precipitate. Preservatives added to the solution may prevent bacterial growth. Please view the data sheet below: https://www.braskem.com.br/Portal/Principal/Arquivos/html/boletm_tecnico/PP%20Chemical%20Resistance.pdf

      Helpful?

  8. I am looking for Nonidet 40, but I am unable to find it. Do you sell this product? If not, can you suggest a suitable alternate product?

    1 answer

    1. Nonidet 40 or NP40 is no longer commercially available. The Igepal CA-630 (Product No. I8896), is the best substitute product that we can recommend.

      Helpful?

  9. Are the 2 products, Igepal CA-630 (Product No. I8896) and Nonidet 40 the same? How do you use this product?

    1 answer

    1. Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.

      Helpful?

  10. What does "Molecular Biology Grade" mean?

    1 answer

    1. Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.

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1–10 of 17 Questions  

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