The proprietary information ont his product is not available
价格与库存信息目前不能提供
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应用
柠檬酸盐缓冲液，pH 6.0，10×，抗原修复液，在应用抗体或DNA荧光原位杂交之前，已被用作福尔马林固定的石蜡包埋(FFPE)组织切片上的热诱导抗原修复液。[1]在免疫组化(IHC)中，最常用的固定剂（如福尔马林）通过其内在交联掩盖组织抗原（细胞、膜和核）。这种掩盖导致 IHC（免疫组化）染色差或无染色。在FFPE组织切片上使用柠檬酸盐缓冲液（pH 6.0）或其他抗原修复液改善了抗体对组织抗原的可及性。
制备说明
将柠檬酸盐缓冲液，pH 6.0，10x，抗原修复液用水稀释 10 倍，以制备1x 工作溶液，例如用 90mL水稀释 10mL 10x 浓缩液。
储存及稳定性
将产品储存在2-8°C。不要冻存，以避免产生沉淀。
其他说明
有时缓冲液可能含有淡黄色。这是由防腐剂引起的，不会影响缓冲液的功能。
将制备的1′ 柠檬酸盐缓冲液，pH 6.0，工作溶液储存于2-8℃。
将制备的1′ 柠檬酸盐缓冲液，pH 6.0，工作溶液储存于2-8℃。
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产品编号
说明
价格
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Can you provide the composition of C9999-1000? Specifically, does it contain any detergents such as Tween?
1 answer-
Helpful?
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Is this a mixture from trisodium citrate and citric acid? I have looked through the SDS and the mixture of the chemical is <0.025% of 5-Chloro-2-methyl-4-isothiazolin-3-one and another chemical. I can't seem to find how they would make up a citrate buffer
1 answer-
The specific formulation is considered proprietary, however, the final citrate concentration is 0.1M. The SDS lists only the potentially hazardous ingredients. The 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-2H-isothiazol-3-one (3:1) listed is essentially ProClin which acts as a preservative. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/748/563/c9999pis.pdfHelpful?
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What molarity does this buffer have?
1 answer-
Product C9999 is provided at a concentration of 0.1 M. It'll be 10 mM when diluted. The solution contains 116.25 mM Tri-NaCitrate, 10.25 mM EDTA, 0.5 % ProClin, and other proprietary materials.
Helpful?
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Is this Citrate buffer already included Tween 20?
1 answer-
The composition of this buffer is considered proprietary. However, citrate solutions commonly used for antigen retrieval do not contain detergents. This product does contain a preservative which may contribute to the color of the solution. This does not effect the performance of the product in any way. Please see the link below to reivew a general antigen retrival protocol using Citrate Buffer. Note that Tween 20 is used in the wash solution following treatment with Citrate Buffer.
IHC World
https://www.ihcworld.com/_protocols/epitope_retrieval/citrate_buffer.htmHelpful?
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