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Merck

C6295

Sigma-Aldrich

细胞冷冻培养基-DMSO 无血清 1x

sterile-filtered, suitable for cell culture

别名:

cryopreservation medium

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75
价格与库存信息目前不能提供

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable
cryopreservation: suitable

杂质

endotoxin, tested

运输

dry ice

储存温度

−20°C

一般描述

细胞冷冻培养基是防冻剂DMSO与胎牛血清的组合。但无血清培养基可用甲基纤维素和泊洛沙姆188以及其他添加剂(如抗坏血酸、α-生育酚和脯氨酸)替代血清。[1]

应用

DMSO无血清1x细胞冷冻培养基已用于冷冻人类神经祖细胞(hNPCs)[2],嗅鞘细胞(OECs)[3]和视网膜色素上皮细胞(RPE)。[4]

其他说明

含 MEM、二甲基亚砜 (8.7%) 和甲基纤维素的完全即用型培养基。根据 Waymouth[5] 的配方配制。

相关产品

产品编号
说明
价格

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Chen-Yang Meng et al.
Oncology reports, 43(4), 1169-1186 (2020-04-24)
Osteosarcoma (OS) is the most common primary malignant tumor of the bone affecting children and adolescents. Chemotherapy is now considered as a standard component of OS treatment, not only for children, but also for adults. However, chemoresistance continues to pose
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
Kai Pollard et al.
Scientific data, 7(1), 184-184 (2020-06-21)
Nerve sheath tumors occur as a heterogeneous group of neoplasms in patients with neurofibromatosis type 1 (NF1). The malignant form represents the most common cause of death in people with NF1, and even when benign, these tumors can result in

商品

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview of good cell banking practices for cell line cryopreservation purposes.

Overview of good cell banking practices for cell line cryopreservation purposes.

用于细胞系冷冻保存的良好细胞库操作实践概述。

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Questions

  1. Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.

    1 answer
    1. The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf

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