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C2999

Sigma-Aldrich

CryoStor® 细胞冻存培养基

CS5

别名:

cell freezing medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

100

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable
cryopreservation: suitable

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

CryoStor CS2、CS5 和 CS10 系列保存溶液代表了下一代冷冻保存培养基。CryoStor 培养基设计用于在超低温环境（-80 至 -196°C）内制备和保存细胞，在冷冻、储存和解冻过程中为细胞和组织提供安全的保护环境。通过调节冻存过程中的细胞生化反应，这些培养基提供了增强的细胞活力和功能性，同时不需要添加血清、蛋白质或高水平的细胞毒性试剂。

Cryostor CS2、CS5 和 CS10 是一系列细胞特异性的、优化的冻存培养基，其独特配方可解决细胞在冻存过程中的分子生物学问题，从而直接降低冻存诱导的迟发性细胞死亡的水平，提高解冻后细胞的活力和功能。

这些培养基被推荐用于冻存干细胞、肝细胞、组织样本和其他非常敏感的细胞类型。

應用

CryoStor 是一系列细胞特异性的、优化的冻存培养基，具有独特的配方，可在冻存过程中解决细胞的分子生物学问题，从而直接降低冻存诱导的迟发性细胞死亡，并提高解冻后细胞的活力和功能。
Cryostor CS5含5％二甲基亚砜（DMSO）。推荐大多数细胞类型选择其用于超低温保藏。

Cryostor® 冷冻保存视频操作流程

其他說明

配方含有5% DMSO。

法律資訊

CryoStor is a registered trademark of BioLife Solutions, Inc.

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Hui Li et al.
Journal of virology (2021-03-05)
Previously, we showed that substitution of HIV-1 Env residue 375-Ser by bulky aromatic residues enhances binding to rhesus CD4 and enables primary HIV-1 Envs to support efficient replication as simian-human immunodeficiency virus (SHIV) chimeras in rhesus macaques (RMs). Here, we
Dominic M Clarke et al.
Cytotherapy, 11(4), 472-479 (2009-06-06)
Peripheral blood stem cells (PBSC) have become the preferred stem cell source for autologous hematopoietic transplantation. A critical aspect of this treatment modality is cryopreservation of the stem cell products, which permits temporal separation of the PBSC mobilization/collection phase from
Ryan S Roark et al.
Science (New York, N.Y.), 371(6525) (2020-11-21)
Neutralizing antibodies elicited by HIV-1 coevolve with viral envelope proteins (Env) in distinctive patterns, in some cases acquiring substantial breadth. We report that primary HIV-1 envelope proteins-when expressed by simian-human immunodeficiency viruses in rhesus macaques-elicited patterns of Env-antibody coevolution very
J Stylianou et al.
Cytotherapy, 8(1), 57-61 (2006-04-22)
Hematopoietic stem cells (HSC) have traditionally been frozen using the cryoprotectant DMSO in dextran-40, saline or albumin. However, the process of freezing and thawing results in loss of HSC numbers and/or function. This study investigated the use of CryoStor for
Haichen Niu et al.
Brain pathology (Zurich, Switzerland), 30(6), 1102-1118 (2020-07-18)
Olfactory dysfunction is one of the early symptoms seen in Parkinson's disease (PD). However, the mechanisms underlying olfactory pathology that impacts PD disease progression and post-mortem appearance of alpha-Synuclein (α-Syn) inclusions in and beyond olfactory bulb in PD remain unclear.

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Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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实验方案

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

CryoStor® Cryopreservation Protocol

Cryopreservation affects post-thaw recovery, viability, and functionality. Stress during freezing and suboptimal media lead to cell death.

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