C2780

软骨素酶AC 来源于肝素黄杆菌

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

• 别名: 软骨素AC裂解酶
• CAS号: 9047-57-8
• EC 号: 4.2.2.5 (BRENDA, IUBMB)
• MDL编号: MFCD00130796
• UNSPSC代码: 12352204
• NACRES: NA.54

属性

• 偶联物: (Glucosaminoglycan)
• 质量水平: 200
• 表单: lyophilized powder
• 比活: 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
• 组成: Protein, ~15% Lowry
• 溶解性: 0.02 M phosphate buffer: soluble (pH 7.0)
• 异质活性: Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
• 储存温度: −20°C

说明

应用

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.

Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

生化/生理作用

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

其他说明

Contains potassium phosphate buffer salts and BSA as stabilizer.

查看有关 用于复合碳水化合物分析的酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

适用性

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

单位定义

One unit will cause a ΔA₂₃₂ of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

制备说明

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)