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Merck
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C0954

Sigma-Aldrich

Chondroitinase C from Flavobacterium heparinum

lyophilized powder, ≥200 units/mg solid

别名:

Chondroitin C lyase

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About This Item

CAS号:
60184-91-0
MDL编号:
MFCD00212904
UNSPSC代码:
12352204
NACRES:
NA.54

偶联物

(Glucosaminoglycan)

质量水平

200

表单

lyophilized powder

比活

≥200 units/mg solid

储存温度

−20°C

相关类别

一般描述

Enzyme Commission (EC) Number 4.2.2.x

生化/生理作用

Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.

单位定义

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.

其他说明

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
027K3790
088H37803
088H37802
076H3845
088H3780

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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities

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