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Merck

B9285

Sigma-Aldrich

5-溴-2′-脱氧尿苷

BioUltra, ≥99%

别名:

5-BrdU, 5-溴-1-(2-脱氧-β-D-呋喃核糖)尿嘧啶, 5-溴脱氧尿苷, BUdR

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About This Item

经验公式(希尔记法):
C9H11BrN2O5
CAS号:
分子量:
307.10
Beilstein:
30395
EC號碼:
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.51
价格与库存信息目前不能提供

生物源

synthetic (organic)

品質等級

產品線

BioUltra

化驗

≥99%

形狀

powder

雜質

≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

燃燒殘留物

≤0.1%

mp

191-194 °C (dec.) (lit.)

溶解度

NH4OH: 0.1 M at 20 °C, clear, colorless

正離子痕跡

Al: ≤0.0005%
Ca: ≤0.01%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

儲存溫度

−20°C

SMILES 字串

OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O

InChI

1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1

InChI 密鑰

WOVKYSAHUYNSMH-RRKCRQDMSA-N

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一般說明

5′-溴-2-脱氧尿苷(BrdU)是一种可被皮肤吸收或吸入的致癌物质。BrdU溶液对光敏感。[1]

應用

5-溴-2′-脱氧尿苷已用于:
  • 在细胞增殖测定中标记妊娠小鼠的增殖细胞[2]
  • 研究缺血后不同时间的细胞增殖时程[3]确认MKI67 +细胞在细胞周期中重新进入生殖细胞[4]
  • 成纤维细胞5′-溴-2′脱氧尿苷(BrdUrd)染色[5]

生化/生理作用

5′-溴-2-脱氧尿苷(BrdU)染色主要用于体内观察肿瘤细胞和其他组织的增殖。[6]
5-溴-2′-脱氧尿苷(5-BrdU)是一种胸苷类似物,可掺入DNA中。 5-BrdU通常广泛用于测定DNA合成和标记分裂细胞。 因此,5-BrdU常用于研究细胞信号传导和诱导细胞增殖的其他过程。
用作遗传研究中诱变剂的胸苷类似物。在 S 期可选择性结合到细胞 DNA 中。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Muta. 1B - Repr. 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 2

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Retinoic Acid Induces Multiple Hallmarks of the Prospermatogonia-to-Spermatogonia Transition in the Neonatal Mouse
Busada JT, et al.
Biology of Reproduction, 90(3) (2014)
Histological, cellular and behavioral assessments of stroke outcomes after photothrombosis-induced ischemia in adult mice
Li H, et al.
BMC Neuroscience, 15(1), 1-13 (2014)
Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene
Dackor RT, et al.
Molecular and Cellular Biology, 26(7), 2511-2518 (2006)
Identification of MYC-Dependent Transcriptional Programs in Oncogene-Addicted Liver Tumors
Kress TR, et al.
Cancer research, 76(12), 3463-3472 (2016)
Non-canonical Cyclic Nucleotides (2017)

Questions

1–5 of 5 Questions  
  1. For how long is Product B9285, 5-Bromo-2´-deoxyuridine, stable in solution?

    1 answer
    1. 5-Bromo-2´-deoxyuridine is stable in solution for 6 months when stored -20°C.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. In what solvents is Product B9285, 5-Bromo-2´-deoxyuridine, soluble?

    1 answer
    1. Solubility information for 5-bromo-2´-deoxyuridine can be found on the product information sheet (under Documents, above).

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  4. What is the shelf life of Product No. B9285 (5-bromo-2´-deoxyuridine)?

    1 answer
    1. Sigma-Aldrich does not assign an expiration date to this product; it is guaranteed for one year from the date of shipment.

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  5. Can Product B9285, 5-Bromo-2´-deoxyuridine, be injected into animals?

    1 answer
    1. Sigma has not injected animals with solutions of this compound, so we do not have a stepwise procedure for you.  However, this application has been reported in the literature.  Please see the cited references below, which you may find useful. Several references cite the use of Product No. B9285 in their protocols.1. Yin, X-X, et al., Expression of active caspase-3 in mitotic and postmitotic cells of the rat forebrain. J. Comp. Neurol., 433(1), 4-22 (2001).  Injected into mice at 50 mg/kg.2. Dackor, R.T., et al., Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene. Mol. Cell. Biol., 26(7), 2511-2518 (2006).  Injected into mice at 100 mg/kg.3. Farris, S.M., et al., Larval and Pupal Development of the Mushroom Bodies in the Honey Bee, Apis mellifera. J. Comp. Neurol., 414, 97-113 (1999).  A 25 mg/mL solution was injected into bee larvae, prepupae, and pupae.4. Gaiano, N., et al., Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain. Neuron, 26(2), 395-404 (2000).  A 20 mg/mL solution was injected into mice.

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