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Merck

A7054

Sigma-Aldrich

ACCUSPIN 系统-Histopaque®-1077

sterile-filtered, density: 1.077 g/mL

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

aseptically filled

形狀

solution

儲存期限

Expiry date on the label.

包裝

pkg of package 12 tubes

密度

1.077 g/mL

應用

hematology
histology

儲存溫度

2-8°C

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一般說明

ACCUSIN System-Histopaque-1077产品用于分离淋巴细胞和其他单核细胞。分离介质Histopaque-1077是一种无菌过滤的、经内毒素检测的聚蔗糖和泛影酸钠溶液,密度调整为1.077 g/mL。ACCUSPIN系统-Histopaque 1077采用γ辐照的50 mL聚丙烯离心管,专门设计多孔高密度聚乙烯屏障(“筛板”)隔开两个腔室。每管无菌灌装15 mL的Histopaque 1077。 每管可分离15-30 mL抗凝血。

應用

ACCUSIN System-Histopaque 1077使用密度梯度分离介质从全血和骨髓中分离淋巴细胞和其他单个核细胞。Histopaque-1077用于:

  • 人淋巴细胞抗原(HLA)分型
  • T、B和‘裸’淋巴细胞计数前的起始分离。
  • 制备用于细胞培养和细胞毒性试验的纯淋巴细胞悬液。
  • 分离和纯化外周血单个核细胞(PBMC)和骨髓单个核细胞(BMMC)
  • 分离人类淋巴细胞
  • 分离全血中乳癌循环肿瘤细胞(CTC)
ACCUSPIN System-HISTOPAQUE-1077 采用专门设计的离心管,两个腔室由多孔高密度聚乙烯屏障(“熔块”)隔开。下部腔室含有 HISTOPAQUE-1077,可添加抗凝全血而不存在与分离介质混合的风险。离心时,全血通过玻璃料下降,与玻璃料下方的 HISTOPAQUE-1077 接触,使血液成分明显分离。红细胞聚集,粒细胞变得略微高渗,增加其沉降率,导致 ACCUSPIN 管底部出现颗粒。淋巴细胞和其他单核细胞,即单核细胞,保留在血浆-HISTOPAQUE-1077 界面。

特點和優勢

ACCUSPIN System-Histopaque®-1077具有很多优点,例如:
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活性
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • Histopaque 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少 2 年

原則

下部分含有HISTOPAUE-1077,保证添加抗凝全血时不会与分离介质混合。离心后,全血下沉通过筛板,与筛板下方的HISTOPAQUE-1077接触,从而将血液成分完全分离。红细胞聚集,粒细胞变得略微高渗,增加其沉降率并导致ACCUSPIN管底部出现沉淀。淋巴细胞和单核白细胞等其他单核细胞留在血浆-HISTOPAQUE-1077分界面。

數量

包装内含 12 根预填充管

法律資訊

Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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