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Merck
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主要文件

C-44010

Sigma-Aldrich

Lymphocyte Separation Medium 1077

500 ml

别名:

Lymphocyte Medium, Separation Medium for Lymphocytes

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.71
价格与库存信息目前不能提供

表单

aqueous solution

包装

pkg of 500 ml

技术

cell culture | mammalian: suitable

运输

ambient

储存温度

room temp

一般描述

Lymphocyte Separation Medium 1077

应用

Lymphocyte Separation Medium 1077 is intended for the separation of the portion of vital mononuclear cells from whole blood, buffy coats, bone marrow and several other starting materials, e.g. crude cell preparations, by means of low density gradient centrifugation. Lymphocyte separation Medium has a density of 1.077 g/ml at 20°C. It is sterile, ready-to use and based on Ficoll 400 and sodium diatrizoate providing optimal physiological parameters and low cytotoxicity.

质量

All lots of PromoCell Lymphocyte Separation Medium 1077 are subjected to comprehensive quality control tests. Each lot is routinely tested for function and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).

储存及稳定性

Lymphocyte Separation Medium 1077 is light-sensitive! Store the medium at room temperature in the dark immediately after arrival. Do not freeze. If stored properly, the product is stable until the expiry date stated on the label.

传代培养常规

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象形图

Corrosion
GHS05

警示用语：

Warning

危险声明

H290,H315,H319

危险分类

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

储存分类代码

8B - Non-combustible corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Primary Human Mononuclear Cells Culture

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

Questions

1–2 of 2 Questions  

  1. What's the procedure of lymphocytes separation with this media and the quantity used .

    1 answer

    1. This is a product of PromoCell. Please see the instructions for use provided by the manufacturer:
      1. Fill Lymphocyte Separation Medium 1077 in a tube. Use 3 ml for a 15 ml tube and 20 ml for a 50 ml tube.
      2. Hold the tube at a 45° angle and carefully load the cell suspension to be separated on top of the separation medium.
      Note: Perform this step slowly but constantly. Take care that the two phases do not mix and a sharp border is present.
      For best results, use an electrical pipet aid with EX-function allowing the slow and constant efflux of the cell suspension from the pipet.
      3. Centrifuge the tube at 440 x g for 40 minutes at room temperature without brake.
      4. Carefully aspirate most of the supernatant without disturbing the layer of mononuclear cells in the interphase.
      5. Aspirate the ring of mononuclear cells from the interphase using a pipet. Keep the volume as small as possible.
      6. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 360 x g for 10 minutes.
      7. Wash the mononuclear cells with PBS/0.1% HSA or BSA and centrifuge at 200 x g for 10 minutes X 2
      8. The mononuclear cell preparation is ready.

      Helpful?

  2. What percentage of lymphocytes/monocytes, etc. would be expected if they use it on fresh human blood?

    1 answer

    1. When utilizing the Lymphocyte Separation Medium 1077 (cat. #C-44010) on fresh human whole blood, the expected percentage of lymphocytes/monocytes typical for this sample is usually in the range of 70-90% of the enriched PBMCs.

      Helpful?

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