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Merck
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11191

Sigma-Aldrich

Histopaque®-1119

sterile-filtered, density: 1.119 g/mL

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

sterile-filtered

形狀

liquid

儲存期限

Expiry date on the label.

雜質

endotoxin, tested

密度

1.119 g/mL

應用

hematology
histology

儲存溫度

2-8°C

相关类别

一般說明

Histopaque®-1119 是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成，经调整密度为 1.119 g/mL，pH 为 8.8–9.0。

應用

Histopaque®-1119适合与Histopaque-1077一起使用，因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。

特點和優勢

Histopaque®-1119 有许多优势，比如：
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

原則

该过程涉及在 Histopaque-1119 上使等体积的 Histopaque-1077 分层，形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心后，红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119 界面； 而在上部血浆/Histopaque-1077 界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。

法律資訊

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

产品编号
说明
价格

象形圖

Corrosion
GHS05

訊號詞

Warning

危險聲明

H290,H315,H319

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Understanding genetic determinants of a microbial phenotype generally involves creating and comparing isogenic strains differing at the locus of interest, but the naturally existing genomic and phenotypic diversity of microbial populations has rarely been exploited. Here we report use of
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A Bansal et al.
Journal of developmental origins of health and disease, 10(2), 164-175 (2018-10-27)
Exposure to the endocrine disruptor bisphenol A (BPA) is ubiquitous and associated with health abnormalities that persist in subsequent generations. However, transgenerational effects of BPA on metabolic health are not widely studied. In a maternal C57BL/6J mice (F0) exposure model
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