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69585

Sigma-Aldrich

4-甲基伞形酮基 N-乙酰基-β- D -氨基葡萄糖

fluorogenic, ≥99.0% (TLC), powder, suitable for fluorescence

别名:

4-甲基伞形酮-2-乙酰胺基-2-脱氧-β- D -吡喃葡萄糖苷

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About This Item

经验公式（希尔记法）:
C18H21NO8
CAS号:
37067-30-4
分子量:
379.36
Beilstein:
1693397
EC 号:
253-333-0
MDL编号:
MFCD00150977
UNSPSC代码:
12352204
PubChem化学物质编号:
57652199
NACRES:
NA.32
价格与库存信息目前不能提供

产品名称

4-甲基伞形酮基 N-乙酰基-β- D -氨基葡萄糖, suitable for fluorescence, ≥99.0% (TLC)

质量水平

100

方案

≥99.0% (TLC)

表单

powder

杂质

≤0.1% free 4-methylumbelliferone

溶解性

DMF: 20 mg/mL, clear, colorless

荧光

λex 317 nm (pH 10.0)
λex 365 nm; λem 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase)

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

[H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1

InChI key

PAVCYMSNMRWMAK-DMYIEBNJSA-N

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应用

4-甲基伞形酮N-乙酰-β-D-氨基葡萄糖苷在酸性几丁质酶活性测定中用作荧光底物。[1]它还用于测定水样滤液中β-N-乙酰己糖胺酶(NAGase)的总活性。[2]

生化/生理作用

4-甲基伞形酮-N-乙酰-β-D-氨基葡萄糖苷(4-MUF-NAG)是一种用于氨基己糖苷酶的合成不带电荧光底物。[3]它由几丁质单体（N-乙酰-β-D-氨基葡糖苷）和4-甲基伞形酮（7-氢氧基-4-甲基香豆素）(4-MUF)组成。4-MUF-NAG用于通过测定β-N-乙酰己糖胺酶(NAGase)活性来检测和估计真菌的生长。[4]

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
β-N-乙酰氨基己糖苷酶的测定[5]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCJ9711
BCCH6626
BCCJ3278
BCCJ5309
BCCJ5304

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Population and biomarker responses of Daphnia magna towards anticholinesterase exposures
Lang Q and Li S
Cogent Biology, 5, 1616363-1616363 (2019)
W He et al.
Prenatal diagnosis, 14(1), 17-22 (1994-01-01)
A new fluorogenic substrate, 4-methylumbelliferyl beta-D-glucosaminide, was used for the assay of acetyl CoA:glucosaminide N-acetyltransferase in chorionic villi, cultured villus cells, and amniocytes. Optimal conditions for the assay and the ranges of enzyme activity were established for the various types
Nick Konkol et al.
Journal of microbiological methods, 80(2), 178-182 (2009-12-23)
A wide variety of cultural heritage materials are susceptible to fungal deterioration. The paper, canvas, and stone constituents of our cultural heritage are subjected to harmful physical and chemical processes as they are slowly consumed by fungi. Remediation of fungal
R M Clegg et al.
Biochemistry, 22(20), 4797-4804 (1983-09-27)
Temperature-jump relaxation methods have been used to study the binding kinetics of fluorescent 4-methylumbelliferyl glycosides of N-acetyl-beta-D-glucosamine and its beta (1 leads to 4)-linked di- and trisaccharides with wheat germ agglutinin. The mono- and disaccharide derivatives yielded biexponential progress curves.
M G Ribeiro et al.
Journal of inherited metabolic disease, 14(5), 715-720 (1991-01-01)
GM2-gangliosidosis B1 variant is thought to be a rare disorder with a wide geographical and ethnic distribution. We report the biochemical findings obtained in different specimens from a group of 11 B1 variant patients originating from the north of Portugal.
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