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Merck

32922

Sigma-Aldrich

Lugol 溶液

according to Lugol

别名:

碘/碘化钾溶液

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About This Item

MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
MA.02
价格与库存信息目前不能提供

形狀

liquid

品質等級

品質

according to Lugol

密度

1.006 g/cm3 at 20 °C

應用

diagnostic assay manufacturing
food and beverages
hematology
histology

儲存溫度

room temp

SMILES 字串

[K+].[I-].II

InChI

1S/I2.HI.K/c1-2;;/h;1H;/q;;+1/p-1

InChI 密鑰

XUEKKCJEQVGHQZ-UHFFFAOYSA-M

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一般說明

Lugol溶液是一种即用型溶液,由溶于4%碘化钾中的6%碘组成。在细菌学中,它通常用作革兰氏染色的媒染剂。[1]它通过固定、染色、复染和封装细菌标本材料,对细菌目标结构染色以进行诊断。

應用

  • 在革兰氏染色中,Lugol溶液通常 媒染剂,以及用于固定无脊椎动物的血细胞 和原生动物。[1]
  • 它适合用于检测植物中的淀粉。
  • 在一项研究中,它被用来评估在不放大图像的情况下, 使用窄带成像技术检测高级别发育不良和 粘膜内食管鳞状细胞癌的效果。
  • 在另一项研究中,它被用来确定在辅助检测、 确定食管小鳞状细胞癌的存在和扩散方面的 优势。

生化/生理作用

碘 能够迅速扩散穿过组织的亲水和疏水区域, 并与蛋白质松散结合,将它们染成棕色。它会不可逆地与 不饱和脂质键以及酪氨酸和组氨酸的芳香环发生反应。[1]

特點和優勢

  • 可直接使用的 溶液。

原則

在细菌学中,革兰氏染色法可以快速区分革兰氏阳性菌和革兰氏阴性菌。细菌壁的胞壁质结构是颜色亲和力的基础。首先用初级染料(结晶紫)给细菌染色。之后加入Lugol溶液作为媒染剂,形成结晶紫-碘复合物。在脱色过程中,这些复合物保留在革兰氏阳性菌细胞壁的多层肽聚糖结构中,呈现蓝紫色。 而革兰氏阴性菌的细胞壁为单层结构,不能保留染料-碘复合物,于是用番红溶液复染,呈现粉红色。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Histological and Histochemical Methods: Theory and Practice
J.A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jason M Cole et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(25), 6335-6340 (2018-06-07)
In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources

Questions

1–3 of 3 Questions  
  1. Which Lugol`s solution for phytoplankton preservation sample used?

    1 answer
    1. This product is not suitable for preserving or fixing phytoplankton. No strong iodine solutions are offered for sale due to regulatory issues. Only weak solutions of iodine are available, generally containing about 1% combined iodine and potassium iodide. Strong solutions suitable for parasitology or for use in preserving phytoplankton are not available.

      Helpful?

  2. Buenas tardes. Por favor, pueden especificar las concentraciones de I2 y KI en las siguientes soluciones de Lugol (32922, 62650, y L6146)? No encuentro la información en su página web. Gracias

    1 answer
    1. The composition of product 32922 is considered proprietary. The exact percentages of I2 and KI cannot be stated. The SDS for 32922 does state the composition for the Iodine is between 0.1% to less than 1%. This would suggest the composition of 32922 is comparable other standard "weak Lugol" formulations. Products L6146 and 62650 can be classified as "weak Lugol" formulations. Product L6146 and 62650 are formulated to contain approximately 0.35% Iodine and 0.70% Potassium Iodide. While the concentration of L6146 is a bit higher at 0.37% Iodine and 0.74% Potassium Iodide, there is no functional difference between these two products.

      ​​​​​​​There are "strong" Lugol formulations that can approach a combined I2 and KI concentration of between 10 to 15%. These "strong" formulations are intended for use in procedures for parasitology. 32922, 62650 and L6146 are not suitable for use in procedures for parasitology.

      Helpful?

  3. What are the differences between Lugol solution, products 32922 & 62650?

    1 answer
    1. The composition of product 32922 is provided in the Certificate of Analysis, while for product 62650, only the density is reported. The formulations of the two products appear to be the same, but there is a difference in the amount of quality control testing performed and reported. Both products are intended for Research Use Only and should be viewed as "weak" Lugol Iodine solutions.

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