04743784001

GC-RICH PCR System, dNTPack

suitable for PCR, hotstart: no, Difficult Templates/Specialty Enzymes PCR

• 别名: GC-rich PCR
• 分類程式碼代碼: 41106300
• NACRES: NA.21

属性

• 用途: sufficient for ≤50 reactions
• 特點: Difficult Templates/Specialty Enzymes PCR; dNTPs included; hotstart: no
• 包裝: pkg of 100 U
• 製造商／商標名: Roche
• 參數: 72 °C optimum reaction temp.
• 技術: PCR: suitable
• 輸入: purified DNA
• 運輸包裝: dry ice
• 儲存溫度: −20°C (−15°C to −25°C)

说明

一般說明

The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.

應用

The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.

特點和優勢

• Ease access to difficult templates:-

including GC-rich targets and repetitive sequences.

• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.
• Cost-effective:-

use the convenient premixed solution of PCR grade NTPs.

包裝

1 kit containing 6 components

品質

The GC-RICH PCR System is function-tested, by amplifying a human 284 bp ApoE genomic fragment, using the GC-RICH PCR System protocols.

單位定義

Volume Activity: 2 U/μl

準備報告

Working concentration: The optimal enzyme concentration range from 0.5 to 5 U per assay. For a standard 50 μl PCR, we recommend using 2 U of the enzyme blend.

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

仅试剂盒组分

• GC-RICH Enzyme Mix, in storage buffer
• GC-RICH PCR Reaction Buffer, with 7.5 mM MgCl₂ and DMSO 5x concentrated
• GC-RICH Resolution Solution 5 M
• MgCl₂ Stock Solution 25 mM
• Water, PCR Grade
• PCR Nucleotide Mix

安全信息

• 危險聲明: H412
• 防範說明: P273 - P501
• 危險分類: Aquatic Chronic 3
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash