OP10L

Anti-c-Myc (Ab-1) Mouse mAb (9E10)

lyophilized, clone 9E10, Calbiochem^®

• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 9E10, monoclonal
• 形狀: lyophilized
• 不包含: preservative
• 物種活性: rat (weakly), human, mouse (weakly)
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 同型: IgG1
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MYC(4609) ; mouse ... Myc(17869) ; rat ... Myc(24577)

说明

一般說明

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.

Recognizes a ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.

This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).

免疫原

Epitope: within amino acids 410-419

Human

a synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc

應用

Flow Cytometry (20 µg/ml or use Cat. No. OP10F)

Frozen Sections (2-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)

Immunoprecipitation (1 µg/sample)

Chromatin Immunoprecipitation (5 µg/ml, see application references)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from a volatile buffer, 100 µg BSA.

重構

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

分析報告

Negative Control
HT1080 cells

Positive Control
HL-60 cells or lung carcinoma

其他說明

LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen.20, 361.
Nisen, P.D., et al. 1986. Cancer Res.46, 6217.
Nau, M.M., et al. 1985. Nature318, 69.
Persson, H., et al. 1984. Science225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 1707.

The c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1