U7752

Urease aus Canavalia ensiformis (Jack bean)

• CAS-Nummer: 9002-13-5
• EC-Nummer: 3.5.1.5 (BRENDA, IUBMB)
• EG-Nummer: 232-656-0
• MDL-Nummer: MFCD00070858
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Canavalia ensiformis
• Qualitätsniveau: 200
• Form: solid
• Mol-Gew.: hexamer 545 kDa; trimer ~272 kDa
• Enthält: ~20% dithiothreitol
• Eignung: suitable for marker for electrophoresis (non-denaturing PAGE)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Molekulargewicht der Untereinheit: ~90,770
Besteht aus sechs Untereinheiten mit einem Molekulargewicht von ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria. It belongs to the superfamily of amidohydrolases and phosphotriesterases. Urease is also found in leguminous seeds.

Anwendung

Urease from Canavalia ensiformis (Jack bean) has been used:

• as a component of the molecular mass standard in electrophoresis
• as a component of the standard in size-exclusion chromatography
• as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem./physiol. Wirkung

Urease is an essential enzyme of the nitrogen cycle. It plays a role in degrading urea into ammonia and carbamate. Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.

Sonstige Hinweise

Contains approx. 1 mg protein

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1