L4385
α-Lactalbumin from bovine milk
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $74.00 |
About This Item
Biological source:
bovine milk
Form:
lyophilized powder
Technique(s):
microbiological culture: suitable
Concentration:
0.9—1.4 mg per vial protein (biuret)
$74.00
Check Cart for Availability
biological source
bovine milk
Quality Segment
form
lyophilized powder
mol wt
~14.2 kDa
concentration
0.9—1.4 mg per vial protein (biuret)
technique(s)
microbiological culture: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... LALBA(281894)
General description
α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.[1]
Application
α-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species.[1] It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.[2]
Biochem/physiol Actions
α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form.[3] α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. [4] [5]The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.[6]
Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase.
Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.
Preparation Note
0.9-1.4 mg/mL after reconstitution with 1 mL of water
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine milk | biological source bovine milk | biological source bovine milk | biological source bovine |
| technique(s) microbiological culture: suitable | technique(s) electrophoresis: suitable | technique(s) indirect ELISA: suitable | technique(s) cell culture | insect: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form powder | form lyophilized powder | form powder |
| Gene Information cow ... LALBA(281894) | Gene Information cow ... LALBA(281894) | Gene Information cow ... LALBA(281894) | Gene Information bovine ... LALBA(281894) |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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