T6258

Triosephosphate Isomerase from rabbit muscle

Type X, lyophilized powder, ≥3,500 units/mg protein

• Synonym(e): D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• CAS-Nummer: 9023-78-3
• EC-Nummer: 5.3.1.1 (BRENDA, IUBMB)
• EG-Nummer: 232-774-2
• MDL-Nummer: MFCD00082080
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Typ: Type X
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥3,500 units/mg protein
• Mol-Gew.: calculated mol wt 53.2 kDa
• Zusammensetzung: Protein, 60-90% biuret
• Fremdaktivität: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Anwendung

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Biochem./physiol. Wirkung

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Verpackung

Package size based on protein content

Einheitendefinition

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Physikalische Form

Sulfate-free, contains EDTA and borate buffer salts

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)