G2267

Glyceraldehyd-3-Phosphat-Dehydrogenase aus Kaninchenmuskel

lyophilized powder, ≥75 units/mg protein

• Synonym(e): GAP-DH
• CAS-Nummer: 9001-50-7
• EC-Nummer: 1.2.1.12 (BRENDA, IUBMB)
• EG-Nummer: 232-609-4
• MDL-Nummer: MFCD00131203
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: rabbit muscle
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥75 units/mg protein
• Zusammensetzung: Protein, ≥80% biuret
• Fremdaktivität: 3-Phosphoglyceric phosphokinase ≤0.05%; lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%; triosephosphate isomerase ≤0.1%
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Anwendung

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

• for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
• to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
• in anti-aggregation assays
• in purified GAPDH studies

Biochem./physiol. Wirkung

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Einheitendefinition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physikalische Form

Lyophilized powder containing citrate buffer salts

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)