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G0282

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor from mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(e):

GM-CSF

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About This Item

MDL-Nummer:
MFCD00241918
UNSPSC-Code:
12352202
NACRES:
NA.77

product name

Granulocyte-Macrophage Colony-Stimulating Factor from mouse, GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Biologische Quelle

mouse

Qualitätsniveau

200

Rekombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

Form

lyophilized powder

Wirksamkeit

≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg)

Qualität

endotoxin tested

Mol-Gew.

~15 kDa (125 amino acids including N-terminal methionine)

Verpackung

pkg of 5 and 50 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

≤1 EU/mg

Farbe

white

Löslichkeit

water: soluble, clear, colorless

UniProt-Hinterlegungsnummer

P01587

Lagertemp.

−20°C

Angaben zum Gen

mouse ... CSF2(12981) , Csf2(12981)

Verwandte Kategorien

Biochem./physiol. Wirkung

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physikalische Form

Lyophilized from 10 mM acetic acid plus 250 μg BSA.

Hinweis zur Analyse

The EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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M H Heim
Journal of receptor and signal transduction research, 19(1-4), 75-120 (1999-03-11)
The Jak-STAT pathway was originally discovered through the study of interferon induced intracellular signal transduction. Meanwhile, a large number of cytokines, hormones and growth factors have been found to activate Jaks and STATs. Jaks (Janus Kinases) are a unique class
R C Skoda
Journal of receptor and signal transduction research, 19(1-4), 741-772 (1999-03-11)
The helical cytokines constitute a family of proteins with a common three-dimensional structure. They exert a wide variety of biological effects with a preference for the hematopoietic system. The effects of helical cytokines are mediated by cell surface receptors, which
M A Guthridge et al.
Stem cells (Dayton, Ohio), 16(5), 301-313 (1998-10-10)
The process of ligand binding leading to receptor activation is an ordered and sequential one. High-affinity binding of GM-CSF, interleukin 3 (IL-3), and IL-5 to their receptors induces a number of key events at the cell surface and within the
Vasiliki Kyrargyri et al.
Glia, 63(4), 549-566 (2014-10-10)
Microglia are CNS resident immune cells and a rich source of neuroactive mediators, but their contribution to physiological brain processes such as synaptic plasticity, learning, and memory is not fully understood. In this study, we used mice with partial depletion
A Kelso
Immunology and cell biology, 76(4), 300-317 (1998-09-02)
Cytokines participate in the induction and effector phases of all immune and inflammatory responses. They are therefore obvious tools and targets for strategies designed to promote, inhibit or redirect these responses. However, the complexity of the cytokine network has hindered
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