H6916

Monozytenkolonien-stimulierender Faktor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): MCSF
• MDL-Nummer: MFCD00166591
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Monozytenkolonien-stimulierender Faktor human, M-CSF, recombinant, expressed in HEK 293 cells, suitable for cell culture
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤4 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: dimer 35-40 kDa (glycosylated)
• Verpackung: pkg of 10 μg
• Hersteller/Markenname: HumanZyme
• Methode(n): cell culture | mammalian: suitable
• UniProt-Hinterlegungsnummer: P09603
• Lagertemp.: −20°C
• Angaben zum Gen: human ... CSF1(1435)

Beschreibung

Allgemeine Beschreibung

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Biochem./physiol. Wirkung

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Physikalische Form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Hinweis zur Analyse

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Quellenangabe

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).
2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).
3. Metcalf, D., Blood, 67, 257 (1986).
4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).
5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

Rechtliche Hinweise

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable