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Wichtige Dokumente
B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(e):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.83
Empfohlene Produkte
Produktbezeichnung
BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
Qualitätsniveau
Form
liquid
Lagertemp.
2-8°C
Verwandte Kategorien
Anwendung
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem./physiol. Wirkung
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Vorsicht
Stable at least one year at 2-8 °C.
Physikalische Form
Ready-to-use.
Rechtliche Hinweise
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Sens. 1
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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