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UFC5003

Amicon® Ultra Zentrifugenfilter, 3 kDa MWCO

greener alternative

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa

Synonym(e):

Zentrifugenkonzentrator, 3 kDa MWCO, 0,5 ml Probenvolumen

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Über diesen Artikel

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Pore size:
3 kDa MWCO
Working volume:
≤500 μL , 0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Quality Segment

400

material

polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

0.5 mL sample volume

technique(s)

protein extraction: suitable, protein purification: suitable

L

4.99 cm (1.96 in.)

diam.

10.8 mm

filtration area

1 cm2

volume

0.5 mL

working volume

≤500 μL , 0.5 mL

pore size

3 kDa MWCO

greener alternative category

Re-engineered

shipped in

ambient

General description

Amicon® Ultra 0,5 ml Zentrifugenfiltervorrichtungen bieten eine schnelle Ultrafiltration mit der Möglichkeit für hohe Konzentrationsfaktoren und leichte Konzentratrückgewinnung aus verdünnten und komplexen Probenmatrizen. Zum Beispiel wird Zytochrom C (12,4 kDa) mit einer Anfangskonzentration von 0,25 mg/ml in der Regel zu >95 % zurückgehalten.

Das vertikale Design und die verfügbare Membranoberfläche bieten eine schnelle Probenverarbeitung, hohe Probenrückgewinnung (in der Regel mehr als 90 % der verdünnten Ausgangslösung) und die Möglichkeit für eine 30-fache Konzentration. Die Polarisation der gelösten Stoffe und anschließendes Fouling der Membran werden durch das vertikale Design minimiert und ein physischer Dead Stop in der Filtervorrichtung verhindert das Zentrifugieren bis zur Austrocknung und einen potenziellen Probenverlust. Effiziente Rückgewinnung der konzentrierten Probe (rückgehaltene Spezies) wird durch einen praktischen Schritt mit umgekehrter Rotationsichtung nach dem Sammeln des Filtrats erzielt.

Amicon® Ultra 0,5 ml Vorrichtungen werden nicht steril bereitgestellt und sind nur für den einmaligen Gebrauch vorgesehen. Durch die vertikale Membran und die umgekehrte Rotationsrichtung können die Amicon® Ultra 0,5-ml-Filter eine gute Leistung bei kürzeren Zentrifugierzeiten bieten.
Wir möchten Ihnen umweltverträglichere Alternativprodukte für nachhaltigere Forschungslösungen anbieten. Die innere Filtrationsvorrichtung besteht aus biozirkulärem Material, das auf der Grundlage des ISCC-Massenbilanzierungsverfahrens verwendet wird. Weitere Informationen finden Sie hier .

Application

  • Ultrafiltration mit einer Ultracel®-Membran mit 3.000-Dalton-Molekulargewichtsausschlussgrenze
  • Konzentration von biologischen Proben, die Antigene, Antikörper, Enzyme, Nukleinsäuren oder Mikroorganismen enthalten
  • Aufreinigung von makromolekularen Komponenten, die in Gewebekulturextrakten oder Zelllysaten, Primer-Entfernung, Linkern oder makromolekularen Markierungen aus einer Reaktionsmischung vorkommen, und Proteinentfernung vor der HPLC.
  • Entsalzung, Pufferaustausch und Proteindialyse

Features and Benefits

  • Ultracel® regenerierte Cellulosemembran mit 3-kDa-NMWCO ermöglicht eine Retentatrückgewinnung von mehr als 90 %
  • Konzentriert 500 μl runter auf 15 μl
  • Konzentrationsfaktor 25x bis 30x
  • Hohe Probenrückgewinnung (mehr als 90 % aus verdünnter Ausgangslösung)
  • Kurze Verarbeitungsdauer: 10 bis 30 Minuten
  • Die Fähigkeit für eine umgekehrte Rotationsrichtung bietet konsistente Rückgewinnungen

Physical form

Farbcode: Transparent

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

parameter

0.5 mL sample volume

parameter

2 mL sample volume

parameter

15 mL sample volume

parameter

4 mL sample volume

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

manufacturer/tradename

Amicon® Ultra

material

polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing

material

polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

pore size

3 kDa MWCO

pore size

3 kDa MWCO

pore size

3 kDa MWCO

pore size

3 kDa MWCO

working volume

≤500 μL , 0.5 mL

working volume

≤2 mL

working volume

≤15 mL

working volume

≤4 mL

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Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Verwandter Inhalt

Amicon Ultra遠心式限外ろ過フィルターユニット
Amicon® Pro Affinity Concentration Kit - Protein A
Simultaneous cell lysis and capture using the Amicon®Pro device

E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.

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Global Trade Item Number

SKUGTIN
UFC50032404053252318931
UFC5003BK08436037125850
UFC50030804053252398506
UFC50039604053252615672

Questions

1–9 of 9 Questions  

  1. I centrifuged 200 µL of water (test) for 30min at 14,000g using a 45° fixed-angle rotor. 145 µL passed through, leaving 55 µL on top. How can I reduce this residual volume to 15 µL stated in the product's features? Increasing speed and time didn't help!

    1 answer

    1. Keep using our User Guide for guidance.  A 3 kda membrane is extremely small and every 3 minutes counts in having water pass through the membrane.  One would want to continue spinning at that speed or shortly below.  In time, the initial volume does decrease.  One cannot concentrate water.  A purpose of using water initially is simply to wet the membrane before introducing a real sample in the hopes of decreasing adherence of the membrane to the substance to be concentrated.  One can simply spin at 500 x g for a few moments then discard, then introduce the sample.  

      Helpful?

  2. I would like to use this device to remove protein from human serum. Afterwards I would need to use the filtered serum in cell culture conditions. Is there also a sterile version of this product available? Or can this product safely be autoclaved?

    1 answer

    1. Amicon Ultra devices are used to concentrate substances in liquids. For example, if a protein of interest is 68 kDa, a device with a 30 kDa molecular weight cutoff would be used to concentrate it. The 68 kDa protein would remain on top of the filter and not pass through, while smaller substances might be present in both the filtrate and the retentate. These devices concentrate rather than purify. A sterile version of this product is not available, but a method to sanitize involves adding 70% isopropyl alcohol, spinning for a minute, and then discarding. This product cannot be autoclaved.

      Helpful?

  3. What is the margin of error for the MWCO? I read somewhere the RECOMMENDATION is to use a MWCO 2-3x smaller than your target. But I was wondering what’s the standard deviation from 3 kDa?

    1 answer

    1. For most membranes, the nominal molecular weight limit, also referred to as the molecular weight cut-off, is determined experimentally under a standard set of operating conditions. These analyses typically employ purified globular proteins to serve as markers or indicators of the retention characteristics of an ultrafiltration membrane. An ultrafiltration membrane with a stated nominal molecular weight limit should retain (reject) at least 90% of a globular solute of that molecular weight in daltons. However, for a wider safety margin, the selected cut-off should be well below the molecular weight of the solute to be retained. It is recommended to use a cut-off 2-3 times smaller than the molecular weight of the protein to be concentrated.

      Helpful?

  4. Is this product compatible with organic solvents, such as DMSO or DMF?

    1 answer

    1. The Amicon Ultra products are suitable for use with samples containing up to 10% DMSO and 10% DMF. Exposure to 100% DMSO is not recommended. Unfortunately, the compatibility of these devices with 100% DMF has not been determined.

      Helpful?

  5. Can I order the filter only, coming with no tube part?

    1 answer

    1. Unfortunately, no, this product is not available as a stand alone item. The tubes are available separately under the product UFC50VL96.

      Helpful?

  6. Is the 0.5 ml 3KDa Amicon device (UFC5003) compatible with a solution containing 5% SDS (Sodium Dodecyl Sulfate)?

    1 answer

    1. The Amicon® Ultra centrifugal devices are designed for processing biological fluids and aqueous solutions. Amicon® Ultra Filter Devices are compatible with concentrations of Sodium dodecyl sulfate (SDS)of 0.1% or less. Prior to use, it is important to verify the chemical compatibility of the sample with the device.

      Helpful?

  7. Hi, Do these filters come with expiry dates? If so where would I be able to find them on the product?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  8. How long should I centrifugate at 14.000 g and how long for the reverse spin at 1000 g?

    1 answer

    1. Typical concentration time for the 3kD membrane device is approximately 30 minutes. However, spin times may need to be adjusted to either a shorter or longer time depending on the sample starting volume, initial concentration, composition, and desired minimum volume. The reverse spin is 1000g for 2 minutes. Please review the product user guide at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/340/pr05485-rev0219.pdf

      Helpful?

  9. Hi, how many g's can be used for the ultracell-3K #UFC500396 product?

    1 answer

    1. The recommended speed with a 35 degree fixed angle rotor is 14,000 g with a reverse spin of 1000 g. This information can be found at this link: https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#ultracentrifugal

      Helpful?

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