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AB0100

Sigma-Aldrich

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

Synonym(e):

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.83

product name

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

Form

solution

Qualitätsniveau

200

Verwendung

sufficient for 400 mL working substrate

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Anwendung

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physikalische Form

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Rechtliche Hinweise

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

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