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N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
Synonym(e):
pNPP
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About This Item
Lineare Formel:
O2NC6H4OP(O)(ONa)2
CAS-Nummer:
Molekulargewicht:
263.05
MDL-Nummer:
UNSPSC-Code:
12352204
PubChem Substanz-ID:
NACRES:
NA.83
Empfohlene Produkte
Produktbezeichnung
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
Form
liquid
Qualitätsniveau
Versandbedingung
wet ice
Lagertemp.
−20°C
SMILES String
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChIKey
VIYFPAMJCJLZKD-UHFFFAOYSA-L
Allgemeine Beschreibung
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
Anwendung
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem./physiol. Wirkung
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
Vorsicht
Stable at least one year when stored below 0 °C.
Physikalische Form
Ready-to-use.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
Zielorgane
Kidney,Liver,Blood
Lagerklassenschlüssel
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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