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Wichtige Dokumente
A4187
Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(e):
Rabbit Anti-Goat IgG (whole molecule)–AP
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About This Item
Empfohlene Produkte
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
alkaline phosphatase conjugate
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous glycerol solution
Speziesreaktivität
goat
Methode(n)
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
Versandbedingung
wet ice
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody (diluted 1:15,000) can be used as a secondary antibody for immunoblotting of Sall4. This antibody can also be used in immunohistochemistry (diluted1:400) . Rabbit anti-goat IgG antibody reacts specifically with goat IgG and normal goat serum.
Primary goat antibodies are often used to study target proteins for various clinical and research purposes. Thus, secondary anti-goat antibody conjugated to a detectable substrate can be used to facilitate the accurate detection and localization of target proteins.
Spezifität
Binds all goat Igs
Immunogen
Purified goat IgG.
Anwendung
Anti-Goat IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution.
Physikalische Form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Analysenzertifikate (COA)
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