A1520

α-Amylase Inhibitor

≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase), ≥1000 inhibitor U/mg protein (using human salivary α-amylase), lyophilized powder

• MDL-Nummer: MFCD00130475
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Produktbezeichnung: α-Amylase Inhibitor from Triticum aestivum (wheat seed), Type I, lyophilized powder
• Biologische Quelle: Triticum estivum
• Qualitätsniveau: 200
• Typ: Type I
• Form: lyophilized powder
• Spezifische Aktivität: ≥1000 inhibitor U/mg protein (using human salivary α-amylase); ≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase)
• Zusammensetzung: Protein, 35-65% biuret
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

α-Amylases are endoglycosidases, that are classified into glycosyl hydrolase family 13 (1-3).[1]

Anwendung

α-Amylase Inhibitor from Triticum aestivum (wheat seed) has been used to determine its effects on salivary gland (SG) amylase activity.[2] It has also been used to inhibit the activity from the extract from Bemisia tabaci.[3]

Biochem./physiol. Wirkung

α-Amylases can hydrolyze α-(1,4)-D-glycosidic linkages. It participates in the breakdown of starch and glycogen. Inhibiting this enzyme can be used as a strategy to treat dental caries, diabetes, obesity and periodontal diseases.[4]

Competitive inhibitor of human salivary α-amylase. K_I = 2.9 nM, compared to a K_M of 5.9 mM (calculated per mole of α-1,4-linked maltose residues).

Einheitendefinition

One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C.

Physikalische Form

Lyophilized powder containing buffer salts as sodium phosphate.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)