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Merck

11579681001

Roche

RNase, DNase-free, High Concentration

from bovine pancreas

Synonym(e):

Rnase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
41105600

Biologische Quelle

bovine pancreas

Qualitätsniveau

Form

solution

Spezifische Aktivität

≥30 U/mg

Verpackung

pkg of 1 mg (10 mg/ml)

Hersteller/Markenname

Roche

Konzentration

10 mg/mL

Allgemeine Beschreibung

A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.

Anwendung

High concentration, DNase-free RNase has been used for:
  • Genomic DNA isolation
  • Isolation of plasmid DNA
  • Degradation of RNA for flow cytometry

Qualität

Absence of DNase activity tested according to the current Quality Control procedures.

Einheitendefinition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Angaben zur Herstellung

Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

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This protocol describes the synthesis and characterization of gold nanoparticle-based nanobeacons as a theranostic strategy for the recognition, detection, and inhibition of miRNA and mRNA. This system is designed for an in vitro evaluation of a sequence's silencing potential and later
Alba Font-Tello et al.
Nature protocols, 15(8), 2503-2518 (2020-06-28)
Fixed-tissue ChIP-seq for H3K27 acetylation (H3K27ac) profiling (FiTAc-seq) is an epigenetic method for profiling active enhancers and promoters in formalin-fixed, paraffin-embedded (FFPE) tissues. We previously developed a modified ChIP-seq protocol (FiT-seq) for chromatin profiling in FFPE. FiT-seq produces high-quality chromatin
Artyom A Alekseyenko et al.
Current protocols in molecular biology, 109, 21-21 (2015-01-07)
In order to understand how chromatin complexes function in the nucleus, it is important to obtain a comprehensive picture of their protein, DNA, and RNA components, as well as their mutual interactions. This unit presents a chromatin cross-linking approach (BioTAP-XL)

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