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10109134001

Roche

RNase

from bovine pancreas

Synonym(e):

Rnase

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About This Item

UNSPSC-Code:
12352204

Biologische Quelle

bovine pancreas

Qualitätsniveau

100

Form

dry powder (prepared from crystallized ribonuclease)

Spezifische Aktivität

~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).)

Verpackung

pkg of 500 mg

Hersteller/Markenname

Roche

Versandbedingung

wet ice

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease).
Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Spezifität

Heat inactivation: 1 mM EDTA

Anwendung

RNase has been used in DNA ladder assay.
RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis.

Einheitendefinition

One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance.

Angaben zur Herstellung

Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Methods in molecular biology (Clifton, N.J.), 1476, 143-165 (2016-08-11)
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Lombardi V R, et al.
Experimental and Therapeutic Medicine, 15(3), 2547-2556 (2018)
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