MAB1973
Anti-Integrin α1 Antibody, clone FB12
clone FB12, Chemicon®, from mouse
Synonym(e):
CD49a
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Alle Fotos(2)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
FB12, monoclonal
Speziesreaktivität
human
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG1
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ITGA1(3672)
Spezifität
Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
Anwendung
Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation.
Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2μg/mL (Sato et al., 2005).
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2μg/mL (Sato et al., 2005).
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1.
Zielbeschreibung
130 kDa
Physikalische Form
Format: Purified
Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Hinweis zur Analyse
Control
Microvascular endothelium
Microvascular endothelium
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Leo K Iwai et al.
The Biochemical journal, 454(3), 501-513 (2013-07-05)
Collagen is an important extracellular matrix component that directs many fundamental cellular processes including differentiation, proliferation and motility. The signalling networks driving these processes are propagated by collagen receptors such as the β1 integrins and the DDRs (discoidin domain receptors).
Huifang Xu et al.
PloS one, 7(12), e52209-e52209 (2013-01-04)
The discoidin domain receptors, DDR1 and DDR2, are receptor tyrosine kinases that bind to and are activated by collagens. Similar to collagen-binding β1 integrins, the DDRs bind to specific motifs within the collagen triple helix. However, these two types of
Katarzyna Biadasiewicz et al.
Endocrinology, 152(4), 1458-1469 (2011-02-10)
The basic helix-span-helix transcription factor activating protein (AP)-2α is critically involved in cell-specific hormone expression of syncytializing human trophoblasts. Its role in invasive trophoblast differentiation, however, remains largely elusive. Using RT-PCR, Western blotting, and immunofluorescence of first-trimester placentae, we here
Sung Wook Park et al.
Diabetes, 63(9), 3057-3068 (2014-04-12)
Pericyte loss is an early characteristic change in diabetic retinopathy (DR). Despite accumulating evidence that hyperglycemia-induced angiopoietin 2 (Ang2) has a central role in pericyte loss, the precise molecular mechanism has not been elucidated. This study investigated the role of
M A Sanders et al.
The Journal of biological chemistry, 275(48), 38040-38047 (2000-09-15)
Integrin-initiated extracellular signal-regulated kinase (ERK) activation by matrix adhesion may require focal adhesion kinase (FAK) or be FAK-independent via caveolin and Shc. This remains controversial for fibroblast and endothelial cell adhesion to fibronectin and is less understood for other matrix
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