Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Archives of virology (2015-07-01)

Leonardo Joaquim van Zyl, Falone Sunda, Mark Paul Taylor, Don Arthur Cowan, Marla Iris Trindade

초록

The study of extremophilic phages may reveal new phage families as well as different mechanisms of infection, propagation and lysis to those found in phages from temperate environments. We describe a novel siphovirus, GVE3, which infects the thermophile Geobacillus thermoglucosidasius. The genome size is 141,298 bp (G+C 29.6%), making it the largest Geobacillus spp-infecting phage known. GVE3 appears to be most closely related to the recently described Bacillus anthracis phage vB_BanS_Tsamsa, rather than Geobacillus-infecting phages described thus far. Tetranucleotide usage deviation analysis supports this relationship, showing that the GVE3 genome sequence correlates best with B. anthracis and Bacillus cereus genome sequences, rather than Geobacillus spp genome sequences.

MATERIALS

제품 번호

브랜드

제품 설명

717975

Sigma-Aldrich

Methanol, NMR reference standard

75746

Sigma-Aldrich

Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets

71717

Sigma-Aldrich

Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

71729

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

650609

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

74255

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

L5750

Sigma-Aldrich

Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis

L4509

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

L6026

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

62862

Sigma-Aldrich

Sodium dodecyl sulfate, ≥90% ((Assay))

L3771

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

C7559

Sigma-Aldrich

Chloroform, ≥99%, PCR Reagent, contains amylenes as stabilizer

309435

Sigma-Aldrich

3-Methyl-1-butanol, anhydrous, ≥99%

372978

Sigma-Aldrich

Chloroform, anhydrous, contains amylenes as stabilizer, ≥99%

480150

Sigma-Aldrich

Chloroform, ACS reagent, ≥99.8%, contains amylenes as stabilizer

288306

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

436143

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

439142

Supelco

Chloroform, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

71725

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

RDD040

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri^™

606723

Sigma-Aldrich

Methanol-¹²C, 99.95 atom % ¹²C

632546

Supelco

Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

05030

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

721956

Sigma-Aldrich

Methanol solution, NMR reference standard, 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.

16017

Sigma-Aldrich

Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)

16016

Sigma-Aldrich

Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)

86600

Sigma-Aldrich

Tetraethylammonium borohydride, technical, ≥95% (T)

33517

Sigma-Aldrich

Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%