71725

Sodium dodecyl sulfate

BioUltra, for molecular biology, ≥99.0% (GC)

• 동의어(들): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Linear Formula: CH₃(CH₂)₁₁OSO₃Na
• CAS Number: 151-21-3
• Molecular Weight: 288.38
• Beilstein: 3599286
• EC Number: 205-788-1
• MDL number: MFCD00036175
• UNSPSC 코드: 12161900
• eCl@ss: 39093306
• PubChem Substance ID: 57652353
• NACRES: NA.25

속성

• Grade: Molecular Biology; for molecular biology
• 설명: anionic
• 제품 라인: BioUltra
• 분석: ≥99.0% (GC)
• 양식: powder
• 분자량: micellar avg mol wt 18,000
• 응집 번호: 62
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• 손실: ≤3% loss on drying, 20 °C (HV)
• CMC: 7-10 mM (20-25°C)
• mp: 204-207 °C (lit.)
• 전이 온도: cloud point >100 °C
• solubility: H₂O: 0.1 M at 20 °C, clear, colorless
• density: 0.455 g/cm³
• 음이온 미량물: chloride (Cl^-): ≤200 mg/kg; phosphate (PO₄^3-): ≤1 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• λ: 0.1 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.04; λ: 280 nm A_max: 0.02
• HLB: 40
• 적합성: suitable for electrophoresis
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• InChI key: DBMJMQXJHONAFJ-UHFFFAOYSA-M

설명

일반 설명

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is a versatile anionic surfactant with a broad range of applications in molecular biology, biochemistry, and various industries. Its amphiphilic nature, characterized by a hydrophobic hydrocarbon tail and a hydrophilic sulfate head group, makes it an effective detergent capable of solubilizing and denaturing proteins, lipids, and other hydrophobic molecules.

In protein biochemistry, SDS plays a pivotal role in protein extraction and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its ability to denature proteins by disrupting their secondary and tertiary structures allows for their uniform migration during gel electrophoresis, enabling the separation and analysis of proteins based on their molecular weight. This technique has become an indispensable tool for identifying and characterizing proteins in biological samples.

Beyond its role in protein analysis, SDS also finds utility in nucleic acid extraction and various hybridization techniques. Its detergent properties aid in the lysis of cells and the release of nucleic acids, facilitating their subsequent purification and analysis. Additionally, SDS is employed in hybridization techniques to promote the annealing of complementary nucleic acid strands, enabling the detection and analysis of specific DNA or RNA sequences.

애플리케이션

Anionic detergent

Sodium dodecyl sulfate has been used:

• as an ionic surfactant in polystyrene (PS) particle suspension for surfactant-based experiments
• in phosphate-buffered saline as a component of lysis buffer to homogenize myometrium and leiomyoma tissue
• as a component of lysis buffer to dissolve formazans
• as a component of high-salt nuclear extraction buffer

생화학적/생리학적 작용

Sodium dodecyl sulfate (SDS) serves as an important detergent in the structural biology field and protein folding/unfolding studies. The sulfate groups and alkyl chains of SDS can strongly bind to the positively charged and the hydrophobic residues of proteins. SDS induces the formation of α-helices in a protein structure. At high concentrations, SDS stimulates cooperative unfolding in various proteins such as bovine serum albumin (BSA), hen egg-white lysozyme, -lactalbumin, and -lactoglobulin structures.

특징 및 장점

• Suitable for molecular biology and biochemical research
• BioUltra grade for your sensitive applications
• Free from DNase, RNase, NICKase and protease
• Tested for trace levels of Anions and Cations
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

• Undergoes hydrolysis at high temperatures, especially in acidic medium

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안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H228,H302 + H332,H315,H318,H335,H412
• 예방조치 성명서: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 4.1B - Flammable solid hazardous materials
• WGK: WGK 2
• Flash Point (°F): 338.0 °F
• Flash Point (°C): 170 °C
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges