콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

53823-U

Supelco

Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

동의어(들):

Core-shell (SPP) Fused Core C18 HPLC column

로그인조직 및 계약 가격 보기

크기 선택

1 EA
₩1,312,598

₩1,312,598


예상 입고일2025년 2월 17일세부사항



크기 선택

보기 변경
1 EA
₩1,312,598

About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

₩1,312,598


예상 입고일2025년 2월 17일세부사항


제품명

Ascentis® Express C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L1

제품 라인

Ascentis®

특징

endcapped

제조업체/상표

Ascentis®

포장

1 ea of

파라미터

60 °C temp. range
600 bar max. pressure (9000 psi)

기술

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

길이 × I.D.

10 cm × 2.1 mm

표면적

135 m2/g

불순물

<5 ppm metals

Matrix

Fused-Core particle platform
superficially porous particle

기질 활성군

C18 (octadecyl) phase

입자 크기

2.7 μm

공극 크기

90 Å

작동 pH

2-9

응용 분야

food and beverages

분리 기술

reversed phase

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

애플리케이션

제품 번호
설명
가격

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Francesca Rigano et al.
Journal of separation science, 44(8), 1571-1580 (2021-02-23)
The present work aims to a promising re-utilization of the massive waste derived from the tuna fishing industry, for which by-products can represent more than 50% of the original material. Due to the considerable content in polyunsaturated fatty acids and
Pankaj Partani et al.
Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)
A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within
Babu Rao Chandu et al.
SpringerPlus, 2(1), 194-194 (2013-06-07)
A bioequivalence study was proved of generic Febuxostat 80 mg tablets (T) in healthy volunteers.For this purpose, Authors developed a simple, sensitive, selective, rapid, rugged and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of Febuxostat (FB) in human
Virginia Brighenti et al.
Journal of pharmaceutical and biomedical analysis, 143, 228-236 (2017-06-14)
The present work was aimed at the development and validation of a new, efficient and reliable technique for the analysis of the main non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp) inflorescences belonging to different varieties. This study was designed
Francesco Pio Prencipe et al.
Journal of chromatography. A, 1349, 50-59 (2014-05-27)
The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE)

문서

A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

프로토콜

Supelco는 음용수 내 BPA 측정을 위한 종합적인 시료 조제 및 분석 절차를 개발했습니다.

A comprehensive sample preparation and analytical procedure was developed by Supelco for determining BPA in drinking water.

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

This study demonstrates the analysis of Warfarin in plasma samples utilizing chiral and achiral (reversed-phase) LC-MS and effective sample prep to remove endogenous phospholipids

모두 보기

관련 콘텐츠

Detailed list of mycotoxin analysis products for Food & Beverage manufacturers and testers, ensuring safety standards.

Chromatograms

application for HPLCapplication for HPLCapplication for HPLCapplication for HPLC자세히 표시

질문

1–9 / 9 질문  
  1. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 답변
    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      도움이 되었습니까?

  2. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 답변
    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      도움이 되었습니까?

  3. Can I use Ascentis Express on a UHPLC system?

    1 답변
    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      도움이 되었습니까?

  4. What is the carbon load for Ascentis® Express C18 HPLC Columns?

    1 답변
    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared

      도움이 되었습니까?

  5. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 답변
    1. Nothing special is required to use Ascentis Express HPLC columns.  To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level.  For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).

      도움이 되었습니까?

  6. Can I use Ascentis Express on any type of HPLC system?

    1 답변
    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      도움이 되었습니까?

  7. What is the Department of Transportation shipping information for this product?

    1 답변
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      도움이 되었습니까?

  8. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 답변
    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      도움이 되었습니까?

  9. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 답변
    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.