T7254

N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

• 동의어(들): (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride
• 실험식(Hill 표기법): C₁₄H₂₁ClN₂O₃S · HCl
• CAS Number: 4272-74-6
• Molecular Weight: 369.31
• Beilstein: 7106867
• EC Number: 224-266-4
• MDL number: MFCD00065395
• UNSPSC 코드: 12352202
• PubChem Substance ID: 24278752
• NACRES: NA.77

속성

• 제품명: N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
• 생물학적 소스: synthetic (organic)
• 분석: ≥96% (TLC)
• 양식: powder
• 효능: 10-100 μM effective concentration
• solubility: H₂O: 50 mg/mL
• 저장 온도: −20°C
• SMILES string: Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
• InChI key: YFCUZWYIPBUQBD-ZOWNYOTGSA-N
• 유전자 정보: human ... NFKB1(4790) , NFKB2(4791)

설명

애플리케이션

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:

• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract[1]
• as a protease inhibitor in tissue and cell homogenization[2]
• as a trypsin inhibitor to determine the specific protease activity levels[3]

생화학적/생리학적 작용

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

주의사항

Stable at least two years when stored desiccated at −20°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves