T7201

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

• 동의어(들): Factor IIa
• CAS Number: 9002-04-4
• 효소 위원회 번호: 3.4.21.5 (BRENDA, IUBMB)
• MDL number: MFCD00166053
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: lyophilized powder
• 특이 활성도: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
• 분자량: heavy chain ~31 kDa; light chain ~6 kDa
• 저장 온도: −20°C

설명

일반 설명

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects. Thrombin consists of A-chain and a catalytic B-chain with the catalytic pocket comprising of His57, Asp102, Ser195 and Ser214. It has a fibrinogen recognition site (FRS) and a heparin binding site (HBS) in the C-terminus. The main function of thrombin is cleavage of fibrinogen to fibrin. It coordinates the coagulation cascade and binds to diverse substrates. It coordinates with protease-activated receptors (PARs) to accomplish the physiological functions. High levels of thrombin elicit neurotoxicity in dopaminergic neurons and contribute to the progression of Parkinson′s disease. Altered thrombin levels modulates coagulation pathway in multiple sclerosis. Patients with coronary artery disease (CAD) show elevated levels of thrombin.

애플리케이션

Thrombin from bovine plasma has been used in the cleavage of the maltose binding protein tag in mini-calpain-5 fusion protein and in thrombin/hirudin spiking experiments in serum samples.

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess a novel antiplatelet therapy in acute coronary syndromes.

생화학적/생리학적 작용

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

단위 정의

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

물리적 형태

Lyophilized from saline sodium citrate buffer, pH 6.5

제조 메모

Traditional thrombin products are activated with bovine brain, whereas T7201 is activated with bovine lung and does not contain any bovine brain products.

분석 메모

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

기타 정보

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)