추천 제품
일반 설명
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
애플리케이션
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
특징 및 장점
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
수량
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
법적 정보
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
키트 구성품 전용
제품 번호
설명
- Thrombin Cleavage Buffer, 10× 10 mL
관련 제품
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
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문서
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
프로토콜
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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