SAE0152
Lysozyme from chicken egg white
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥40,000 units/mg protein
동의어(들):
Lysozyme from chicken egg white, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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모든 사진(2)
About This Item
CAS Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.77
추천 제품
일반 설명
Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
애플리케이션
- Lysozyme is used for the extraction of genomic DNA from bacterial cells.
- Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
- Lysonzyme is used to prepare spheroplasts.
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
생화학적/생리학적 작용
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
특징 및 장점
Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
단위 정의
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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