The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
S8830
Protease Inhibitor Cocktail
SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins
동의어(들):
Protease Inhibitor Cocktail
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크기 선택
2 TABS
₩102,008
20 TABS
₩780,941
모든 사진(2)
크기 선택
보기 변경
2 TABS
₩102,008
20 TABS
₩780,941
About This Item
추천 제품
제품명
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
생물학적 소스
bovine lung
양식
tablet
저장 온도
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection.
특이성
Inhibits serine, cysteine, aspartic, and metalloproteases.
애플리케이션
The broad specificity of SIGMAFAST™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select® media.
특징 및 장점
EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
성분
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
수량
One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
물리적 형태
Tablet that is water soluble
저장 및 안정성
The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
법적 정보
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
관련 제품
제품 번호
설명
가격
또한 이 제품과 함께 일반적으로 구입
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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문서
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 답변-
도움이 되었습니까?
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
1 답변-
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 답변-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
도움이 되었습니까?
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 답변-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
도움이 되었습니까?
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What is the difference between Protease Inhibitors P8849 and S8830?
1 답변-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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