539134

Protease Inhibitor Cocktail III

EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.

• 동의어(들): EDTA free Protease inhibitor, EDTA-Free Protease Inhibitor Mix, Protease inhibitor cocktail III
• UNSPSC 코드: 12352200
• NACRES: NA.77
• 양식: liquid
• 저장 온도: −20°C

속성

• 제품명: Protease Inhibitor Cocktail Set III, EDTA-Free, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.
• 양식: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Note: available as 1 x 1 ml or as a set of 5 x 1 ml.

This protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.

특이성

Inhibits a broad spectrum of serine proteases, cysteine proteases, and aspartic proteases, as well as aminopeptidases.

애플리케이션

Protease Inhibitor Cocktail Set III, EDTA-Free has been used:

• as a component in CelLytic MT cell lysis reagent or lysis buffer to homogenize kidney organoids for immunoblot analysis
• as a component in homogenization buffer to homogenize minced mouse quadriceps femoris muscle tissue
• in lysis buffer to extract proteins for immunoblotting

생화학적/생리학적 작용

Protease inhibitor cocktails and specialized protease inhibitors play a crucial role in various proteomic studies conducted by scientists. These studies encompass protein expression and characterization, biomarker discovery, identification of post-translational modifications, protein expression profiling, and quantitative protein measurement. The inclusion of a protease inhibitor cocktail or specific protease inhibitor guarantees the preservation of protein integrity, allowing for accurate downstream analysis and comprehensive sample characterization.

특징 및 장점

• EDTA-Free: recommended for IMAC applications.
• Flexible: compatible with bacterial extracts, mammalian cell extracts, and tissue extracts.
• Fast: no waiting time for tablets to dissolve.
• Economical: ready-to-use format reduces wastage.
• Effective: one ml is sufficient for approximately 20g of tissue.
• Complete: contains six protease inhibitors to inhibit aspartic, cysteine, serine proteases, and aminopeptidases.

경고

Toxicity: Irritant (B)

재구성

Following initial thaw, aliquot and freeze (-20°C).

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319
• 예방조치 성명서: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F - (refers to pure substance)
• Flash Point (°C): 87 °C - (refers to pure substance)